MID1 Antibody
| Code | Size | Price |
|---|
| PSI-28-162-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Fish
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MID1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
MID1, OS, FXY, OSX, OGS1, XPRF, BBBG1, GBBB1, MIDIN, RNF59, ZNFXY, TRIM18
Application Note:
MID1 antibody can be used for detection of MID1 by ELISA at 1:312500. MID1 antibody can be used for detection of MID1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
MID1 is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Several different transcript variants are generated by alternate splicing; however, the full length nature of two variants has not been determined.
Background References:
- Winter, J., et al., (2004) Hum. Genet. 114 (6), 541-552.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MID1.
NCBI Gene ID #:
4281
NCBI Official Name:
midline 1 (Opitz/BBB syndrome)
NCBI Official Symbol:
MID1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa, 75 kDa, 62 kDa
Protein Accession #:
NP_000372
Protein GI Number:
4557753
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription
Swissprot #:
O15344
User NOte:
Optimal dilutions for each application to be determined by the researcher.