POLR2B Antibody
| Code | Size | Price |
|---|
| PSI-27-602-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POLR2B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
POLR2B, RPB2, POL2RB, hsRPB2, hRPB140
Application Note:
POLR2B antibody can be used for detection of POLR2B by ELISA at 1:1562500. POLR2B antibody can be used for detection of POLR2B by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
POLR2B encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
Background References:
- Zhou, M., et al., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (22), 12666-12671.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2B.
NCBI Gene ID #:
5431
NCBI Official Name:
polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
NCBI Official Symbol:
POLR2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
134 kDa
Protein Accession #:
NP_000929
Protein GI Number:
4505941
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription
Swissprot #:
P30876
User NOte:
Optimal dilutions for each application to be determined by the researcher.