YAF2 Antibody
| Code | Size | Price |
|---|
| PSI-27-352-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store YAF2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
YAF2, MGC41856,
Application Note:
YAF2 antibody can be used for detection of YAF2 by ELISA at 1:62500. YAF2 antibody can be used for detection of YAF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
YAF2 interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. YAF2 contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1.The protein encoded by this gene interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two have been confirmed to date.
Background References:
- Madge, B., (2003) Cancer Lett. 193 (2), 171-176.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human YAF2.
NCBI Gene ID #:
10138
NCBI Official Name:
YY1 associated factor 2
NCBI Official Symbol:
YAF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
NP_005739
Protein GI Number:
60218897
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription
Swissprot #:
Q8IY57
User NOte:
Optimal dilutions for each application to be determined by the researcher.