NFAT5 Antibody

ProSci
Product Code: PSI-28-678
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-678-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFAT5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on HT-29 at: 1:1000 (Lane 1: 20ug HT 29 lysate blocked with 5% FBS Lane 2: 20ug HT 29 lysate blocked with no FBS Lane 3: 20ug HT-29 lyaste blocked with no PBS + 100 mM NaCl ).
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Antibody used in WB on HT-29 at: 1:1000 (Lane 1: 20ug HT 29 lysate blocked with 5% FBS Lane 2: 20ug HT 29 lysate blocked with no FBS Lane 3: 20ug HT-29 lyaste blocked with no PBS + 100 mM NaCl ).

Further Information

Additional Names:
NFAT5, CAG-8, CAG80, OREBP, nfatz, NFATL1, TonEBP, AI225870, mKIAA0827, B130038B15Rik
Application Note:
NFAT5 antibody can be used for detection of NFAT5 by ELISA at 1:62500. NFAT5 antibody can be used for detection of NFAT5 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Nfat5 contains 1 RHD (Rel-like) domain, and plays a role in the inducible expression of genes. Nfat5 regulates hypertonicity-induced cellular accumulation of osmolytes. Nfat5 might be capable of forming stable dimers with DNA elements
Background References:
  • Pan, S., et al., (2000) Biochem. Biophys. Res. Commun. 272 (3), 765-776.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse NFAT5.
NCBI Gene ID #:
54446
NCBI Official Name:
nuclear factor of activated T cells 5
NCBI Official Symbol:
NFAT5
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
74 kDa
Protein Accession #:
AAF79117
Protein GI Number:
8777837
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription
Swissprot #:
Q9JIH4
User NOte:
Optimal dilutions for each application to be determined by the researcher.