PUS1 Antibody
| Code | Size | Price |
|---|
| PSI-28-640-100ul | 100ul | £662.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PUS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
PUS1, MPUS1, mPus1p, A730013B20Rik
Application Note:
PUS1 antibody can be used for detection of PUS1 by ELISA at 1:312500. PUS1 antibody can be used for detection of PUS1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Pus1 converts specific uridines to PSI in a number of tRNA substrates. Pus1 acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Pus1 is involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.
Background References:
- Zhao, X., et al., (2004) Mol. Cell 15 (4), 549-558.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PUS1.
NCBI Gene ID #:
56361
NCBI Official Name:
pseudouridine synthase 1
NCBI Official Symbol:
PUS1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
NP_001020733
Protein GI Number:
70906472
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription
Swissprot #:
Q791J1
User NOte:
Optimal dilutions for each application to be determined by the researcher.