APOBEC3A Antibody

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ProSci
Product Code: PSI-46-697
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-697-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-697 (3.8ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-697 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and some cytoplasmic staining. The nuclear
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46-697 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
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46-697 (3.8ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-697 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and some cytoplasmic staining. The nuclear
46-697 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f

Documents

Further Information

Additional Names:
APOBEC3A, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, ARP3, PHRBN, bK150C2.1, phorbolin 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 26-28kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0kDa according to Human NP_663745.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Recommended concentration: 3-6ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Background References:
  • Goila-Gaur R, Khan MA, Miyagi E, Kao S, Strebel K. Targeting APOBEC3A to the viral nucleoprotein complex confers antiviral activity. Retrovirology. 2007 Aug 29;4:61.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TSNFNNGIGRHKTY
NCBI Gene ID #:
200315
NCBI Official Name:
apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
NCBI Official Symbol:
APOBEC3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_663745.1
Protein GI Number:
21955158
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
P31941