APOE Antibody

ProSci
Product Code: PSI-45-267
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-267-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-267 (2ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-267 (2ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
APOE, AD2, MGC1571, apoprotein, apolipoprotein E, apolipoprotein E3, Alzheimer disease 2 (APOE*E4-associated, late onset), LPG
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 36kDa band observed in Human Brain lysates (calculated MW of 36.2kDa according to NP_000032.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Background References:
  • Dodart JC, Marr RA, Koistinaho M, Gregersen BM, Malkani S, Verma IM, Paul SM. Gene delivery of human apolipoprotein E alters brain A{beta} burden in a mouse model of Alzheimer's disease. Proc Natl Acad Sci USA. 2005 Jan 25;102(4):1211-6. Epub 2005 Jan 18.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-VGTSAAPVPSDNH
NCBI Gene ID #:
348
NCBI Official Name:
apolipoprotein E
NCBI Official Symbol:
APOE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000032.1
Protein GI Number:
4557325
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Infectious Disease
SPECIFICITY:
.
Swissprot #:
P02649