ASPN Antibody

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ProSci
Product Code: PSI-42-732
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-732-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-732 (0.1ug/ml) staining of Human Tonsil (A), (0.3ug/ml) Human Uterus (B), Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.
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42-732 (0.1ug/ml) staining of Human Tonsil (A), (0.3ug/ml) Human Uterus (B), Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ASPN, PLAP1, SLRR1C, FLJ20129, asporin (LRR class 1), small leucine-rich protein 1C, periodontal ligament associated protein 1, asporin, OS3, asporin proteoglycan
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in Human Tonsil and Uterus and in Mouse and Rat Skeletal Muscle lysates (calculated MW of 43.3kDa according to Human NP_060150.4, and 42.6kDa acciording to Mouse NP_001165952.1 and Rat NP_001014030.1). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Cerebellum lysate.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-IHENKVKKIQKDT
NCBI Gene ID #:
54829
NCBI Official Name:
asporin
NCBI Official Symbol:
ASPN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060150.4
Protein GI Number:
41350214
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q9BXN1