ATG4D Antibody

ProSci
Product Code: PSI-42-094
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-094-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-094 (2.5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

42-094 (2.5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
ATG4D, ATG4 autophagy related 4 homolog D (S. cerevisiae), APG4-D, APG4D, AUTL4, APG4 autophagy 4 homolog D, AUT-like 4, cysteine endopeptidase, autophagin-4, autophagy-related cysteine endopeptidase 4, cysteine protease involved in autophagy
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_116274.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 2.5ug/ml.
Background References:
  • Mari?o G, Ur?a JA, Puente XS, Quesada V, Bordallo J, L?pez-Ot?n C. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. The Journal of biological chemistry 2003 Feb 278 (6): 3671-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: CSQLAQPTLRLPRT
NCBI Gene ID #:
84971
NCBI Official Name:
autophagy related 4D, cysteine peptidase
NCBI Official Symbol:
ATG4D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_116274.3
Protein GI Number:
27903825
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Autophagy
Swissprot #:
Q86TL0