B3GNT2 Antibody

ProSci
Product Code: PSI-46-631
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-631-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-631 (0.1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-631 (0.1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
B3GNT2, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 , B3GN-T1, B3GN-T2, B3GNT, B3GNT-2, B3GNT1, BETA3GNT, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, beta-1,3-N-acetylglucosaminyltransferase bGnT-1, beta-1,3-N-a
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in Human Pancreas lysates (calculated MW of 46.0kDa according to NP_006568.2). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Hayashi N, Nakamori S, Okami J, Nagano H, Dono K, Umeshita K, Sakon M, Narimatsu H, Monden M. Association between expression levels of CA 19-9 and N-acetylglucosamine-beta;1, 3-galactosyltransferase 5 gene in human pancreatic cancer tissue. Pathobiology. 2004;71(1):26-34.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-EKHKGFRTFDIE
NCBI Gene ID #:
10678
NCBI Official Name:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
NCBI Official Symbol:
B3GNT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006568.2
Protein GI Number:
9845238
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
Q9NY97