BDH2 Antibody

ProSci
Product Code: PSI-46-632
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-632-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-632 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-632 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
BDH2, DHRS6, 3-hydroxybutyrate dehydrogenase, type 2, EFA6R, FLJ13261, PRO20933, UCPA-OR, UNQ6308, dehydrogenase/reductase (SDR family) member 6, oxidoreductase UCPA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum), Kidney and Liver lysates (calculated MW of 26.7kDa according to NP_064524.3). In transfected HEK293 transiently expressing BDH2 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Background References:
  • Guo K, Lukacik P, Papagrigoriou E, Meier M, Lee WH, Adamski J, Oppermann U. Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase. J Biol Chem. 2006 Apr 14;281(15):10291-7. Epub 2005 Dec 27.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-TKKKQIDQFANE
NCBI Gene ID #:
56898
NCBI Official Name:
3-hydroxybutyrate dehydrogenase, type 2
NCBI Official Symbol:
BDH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_064524.3
Protein GI Number:
66933014
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q9BUT1