C4orf14 Antibody

ProSci
Product Code: PSI-42-047
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-047-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
NOA1, nitric oxide associated 1, C4orf14, hAtNOS1, hNOA1, mAtNOS1, nitric oxide associated-1, nitric oxide synthase, mitochondrial (putative), nitric oxide-associated protein 1, putative ortholog of Arabidopsis mitochondrial nitric oxide synthase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Breast and in Breast cancer lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.5kDa according to NP_115689.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Parihar A, Parihar MS, Chen Z, Ghafourifar P. mAtNOS1 induces apoptosis of human mammary adenocarcinoma cells. Life Sci. 2008 May 23;82(21-22):1077-82.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat
Immunogen:
The immunogen for this antibody is: ESDYCTAKGSEAIDR
NCBI Gene ID #:
84273
NCBI Official Name:
nitric oxide associated 1
NCBI Official Symbol:
NOA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_115689.1
Protein GI Number:
14150078
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Apoptosis
Swissprot #:
Q8NC60