Caveolin-3 Antibody

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ProSci
Product Code: PSI-46-992
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-992-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-992 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-992 (0.3ug/ml) staining of Mouse (A) and Rat (B) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-992 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-992 (0.3ug/ml) staining of Mouse (A) and Rat (B) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CAV3, caveolin 3, LGMD1C, LQT9, MGC126100, MGC126101, MGC126129, VIP-21, VIP21, M-caveolin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 23kDa band observed in Human, Mouse and Rat Heart lysates and 20kDa in Pig heart and Skeletal Muscle lysates (calculated MW of 17.3kDa according to NP_203123.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows peripheral staining of fibres. Recommended concentration, 3-5ug/ml.
Background References:
  • Fine SW, Lisanti MP, Argani P, Li M. Caveolin-3 is a sensitive and specific marker for rhabdomyosarcoma. Appl Immunohistochem Mol Morphol. 2005 Sep;13(3):231-6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: EHTDLEAQIVKDIH-C
NCBI Gene ID #:
859
NCBI Official Name:
caveolin 3
NCBI Official Symbol:
CAV3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_203123.1
Protein GI Number:
15451860
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
Reported variants represent identical protein (NP_001225.1; NP_203123.1).
Swissprot #:
P56539