CHRNA4 Antibody
| Code | Size | Price |
|---|
| PSI-42-755-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
CHRNA4, cholinergic receptor, nicotinic, alpha 4, BFNC, EBN, EBN1, NACRA4,cholinergic receptor, nicotinic, alpha 4 subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, neuronal acetylcholine receptor alpha 4 subunit, neuronal nicotinic acetylcholine receptor alpha-4 subunit
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? ~75kDa confirmed in both human and rat tissues as the glycoprotein by an anonymous customer.This antibody was shown unfit for immune precipitation.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HVETRAHAEERLLKK
NCBI Gene ID #:
1137
NCBI Official Name:
cholinergic receptor, nicotinic, alpha 4 (neuronal)
NCBI Official Symbol:
CHRNA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000735.1
Protein GI Number:
4502827
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected NOT to cross-react with the similar alpha 2 subunit.
Swissprot #:
P43681