Clusterin Antibody
| Code | Size | Price |
|---|
| PSI-46-749-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
ApoJ, clusterin, Clu, AI893575, Cli, D14Ucla3, SP-40, Sgp-2, Sgp2, Sugp-2, Apolipoprotein J, complement lysis inhibitor, testosterone repressed prostate message
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 38kDa band observed in Rat Brain and Human Testis lysates.(calculated MW of 51.4kDa according to Rat XP_006252156.1) The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources and the literature (Miyata et al, PMID: 11560857). The 38kDa band is thought to correspond to the alpha chain of APOJ. Recommended concentration: 0.1-1ug/ml. An additional band of unknown identity was also consistently observed at 28kDa in Rat Brain. This band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
- Charnay Y, Imhof A, Vallet PG, Hakkoum D, Lathuilier A, Poku N, Aronow B. Kovari E, Bouras C, Giannakopoulos P. Clusterin expression during fetal and postnatal CNS development in mouse. Neuroscience. 2008 Jun 17.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-EKALQEYRRKSRAE
NCBI Gene ID #:
12759 (mouse); 24854 (rat);
NCBI Official Name:
clusterin
NCBI Official Symbol:
Clu
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_038520.1
Protein GI Number:
7304967
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q06890