COG1 Antibody

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ProSci
Product Code: PSI-46-962
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-962-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-962 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-962 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
COG1, component of oligomeric golgi complex 1, CDG2G, DKFZp762L1710, KIAA1381, LDLB, conserved oligomeric Golgi complex protein 1, low density lipoprotein receptor defect B complementing
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 110kDa band observed in Human Brain (Cerebellum, Amygdala) lysates (calculated MW of 109kDa according to NP_061184.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Vasile E, Oka T, Ericsson M, Nakamura N, Krieger M. IntraGolgi distribution of the Conserved Oligomeric Golgi (COG) complex. Exp Cell Res. 2006 Oct 1;312(16):3132-41.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KAKSTRNIETKAQ
NCBI Gene ID #:
9382
NCBI Official Name:
component of oligomeric golgi complex 1
NCBI Official Symbol:
COG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_061184.1
Protein GI Number:
21237783
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
Q8WTW3