CTLA Antibody
| Code | Size | Price |
|---|
| PSI-46-737-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
CLTA, CTLB, clathrin, light chain (Lca), LCA, clathrin, light polypeptide (Lca), clathrin, light polypeptide A, clathrin, light chain (Lcb), LCB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 2.5ug/ml.
Background References:
- Poupon V, Girard M, Legendre-Guillemin V, Thomas S, Bourbonniere L, Philie J, Bright NA, McPherson PS. Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly. Proc Natl Acad Sci USA. 2008 Jan 8;105(1):168-73. Epub 2007 Dec 28.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CDFNPKSSKQAKD
NCBI Gene ID #:
1211; 1212
NCBI Official Name:
clathrin, light chain A
NCBI Official Symbol:
CLTA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001824.1, NP_009027.1, NP_001070145.1, NP_001825.1, NP_009028.1
Protein GI Number:
4502899
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
SPECIFICITY:
This antibody is expected to recognize all reported isoforms of light chains a and b.
Swissprot #:
P09496