DAG1 Antibody

ProSci
Product Code: PSI-42-607
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-607-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-607 (0.2ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-607 (0.2ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
DAG1, dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4kDa according to NP_004384.4). Approx 80kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 96.9kDa according to NP_034147.1). The observed molecular weights correspond to the glycosylated form of alpha-dystroglycan. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HVGKHEYFMHATDK
NCBI Gene ID #:
1605
NCBI Official Name:
dystroglycan 1 (dystrophin-associated glycoprotein 1)
NCBI Official Symbol:
DAG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004384.4
Protein GI Number:
260099735
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Neuroscience
SPECIFICITY:
This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.
Swissprot #:
Q14118