DLL1 Antibody

ProSci
Product Code: PSI-46-984
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-984-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-984 (2ug/ml) staining of Rat Pancreas lysate (A) + Peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-984 (2ug/ml) staining of Rat Pancreas lysate (A) + Peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
DLL1, delta-like 1 (Drosophila), DELTA1, DL1, Delta, OTTHUMP00000017690, delta (Drosophila)-like 1, delta-like 1, delta-like 1 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Mouse Heart and Rat Pancreas lysates (calculated MW of 78.5kDa according to Mouse NP_031891.2 and 77.4kDa according to Rat NP_114452.1). An additional band of nknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration:0.3-7ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:
  • Ivey KN, Muth A, Arnold J, King FW, Yeh RF, Fish JE, Hsiao EC, Schwartz RJ, Conklin BR, Bernstein HS, Srivastava D. MicroRNA regulation of cell lineages in mouse and human embryonic stem cells. 2008 Mar 2 (3): 219-29.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ATQRHLTVGEEWSQD
NCBI Gene ID #:
28514
NCBI Official Name:
delta-like 1 (Drosophila)
NCBI Official Symbol:
DLL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005609.3
Protein GI Number:
110735443
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Neuroscience
SPECIFICITY:
.
Swissprot #:
O00548