EIF2B4 Antibody

ProSci
Product Code: PSI-42-814
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-814-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-814 (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

42-814 (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
EIF2B4, eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa, DKFZp586J0119, EIF-2B, EIF2B, EIF2Bdelta, eIF-2B GDP-GTP exchange factor subunit delta, eukaryotic translation initiation factor 2B, subunit 4 delta, translation initiation factor eIF-2b delta subunit
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in lysates of cell line K562 (calculated MW of 59.6kDa according to NP_751945.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RKDYGSKVSLFSH
NCBI Gene ID #:
8890
NCBI Official Name:
eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
NCBI Official Symbol:
EIF2B4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_751945.2, NP_001029288.1, NP_056451.3
Protein GI Number:
187607323
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_751945.2; NP_001029288.1; NP_056451.3).
Swissprot #:
Q9UI10