EPB41L4B Antibody

ProSci
Product Code: PSI-46-654
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-654-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-654 (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-654 (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
EPB41L4B, erythrocyte membrane protein band 4.1 like 4B, CG1, DKFZp761N1814, EHM2, FLJ21596, EHM2, FERM-containing protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 48kDa band in Human Brain (Hippocampus, Cerebellar Cortex, Cerebellum) and Human Bone Marrow lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.6kDa according to NP_060894.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75ug/ml.
Background References:
  • Diakowski W, Grzybek M, Sikorski AF. Protein 4.1, a component of the erythrocyte membrane skeleton and its related homologue proteins forming the protein 4.1/FERM superfamily. Folia Histochem Cytobiol. 2006;44(4):231-48. Review.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HNYQPQYHPNIHPSQ
NCBI Gene ID #:
54566
NCBI Official Name:
erythrocyte membrane protein band 4.1 like 4B
NCBI Official Symbol:
EPB41L4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060894.2, NP_061987.3
Protein GI Number:
110624794
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_060894.2; NP_061987.3).
Swissprot #:
Q9H329