ERCC1 Antibody

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ProSci
Product Code: PSI-42-738
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-738-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-738 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-738 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ERCC1, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence), COFS4, UV20, excision repair cross-complementing 1, excision repair protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6kDa according to NP_973730.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: DPGKDKEGVPQPS-C
NCBI Gene ID #:
2067
NCBI Official Name:
excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
NCBI Official Symbol:
ERCC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_973730.1, NP_001974.1
Protein GI Number:
42544169
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).
Swissprot #:
P07992