ERP29 Antibody

ProSci
Product Code: PSI-46-744
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-744-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-744 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

46-744 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
ERP29, endoplasmic reticulum protein 29, C12orf8, ERp28, ERp31, PDI-DB, endoplasmic reticulum lumenal protein ERp28
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 28kDa band observed in Human Liver lysates (calculated MW of 29.0kDa according to NP_006808.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. This product has been successfully used in WB on Rat: Gao J et al, Mol Med Rep. 2016 Jan;13(1):398-402. PMID: 26548430.Immunofluorescence:This product has been successfully used in IF on Rat: Gao J et al, Mol Med Rep. 2016 Jan;13(1):398-402. PMID: 26548430.
Background References:
  • Rainey-Barger EK, Magnuson B, Tsai B. A chaperone-activated nonenveloped virus perforates the physiologically relevant endoplasmic reticulum membrane. J Virol. 2007 Dec;81(23):12996-3004.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EKNKMSDGKKEELQ
NCBI Gene ID #:
10961
NCBI Official Name:
endoplasmic reticulum protein 29
NCBI Official Symbol:
ERP29
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006808.1
Protein GI Number:
5803013
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_006808.1) only.
Swissprot #:
P30040