Ezrin Antibody

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ProSci
Product Code: PSI-45-171
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-171-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-171 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-171 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
villin 2 (ezrin) , ezrin, VIL2, CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584 , cytovillin, villin 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80kDa band observed in Human Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Colon and Kidney. Recommended concentration: 3.75ug/ml.
Background References:
  • Stanasila L, Abuin L, Diviani D, Cotecchia S. Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling. J Biol Chem. 2006 Feb 17;281(7):4354-63. Epub 2005 Dec 13.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-QDYEEKTKKAER
NCBI Gene ID #:
7430
NCBI Official Name:
ezrin
NCBI Official Symbol:
VIL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003370.2
Protein GI Number:
21614499
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
P15311