FBXO32 Antibody

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ProSci
Product Code: PSI-42-062
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-062-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-062 (0.3ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-062 (0.3ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
FBXO32, F-box protein 32, FLJ32424, Fbx32, MAFbx, MGC33610, F-box only protein 32, atrogin 1, muscle atrophy F-box protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 40kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 41.5kDa according to Mouse NP_080622.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Pig Skeletal Muscle, and Pig Heart for this particular batch.
Background References:
  • Hanai J, Cao P, Tanksale P, Imamura S, Koshimizu E, Zhao J, Kishi S, Yamashita M, Phillips PS, Sukhatme VP, Lecker SH. The muscle-specific ubiquitin ligase atrogin-1/MAFbx mediates statin-induced muscle toxicity. J Clin Invest. 2007 Dec;117(12):3940-51.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-NSKTKTQYFHQEK
NCBI Gene ID #:
114907
NCBI Official Name:
F-box protein 32
NCBI Official Symbol:
FBXO32
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_478136.1
Protein GI Number:
17158037
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Stem Cell
SPECIFICITY:
This antibody is expected to recognize reported isoform 1 (NP_478136.1).
Swissprot #:
Q969P5