GABRA4 Antibody

ProSci
Product Code: PSI-46-783
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-783-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-783 (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-783 (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
GABRA4, gamma-aminobutyric acid (GABA) A receptor, alpha 4, gamma-aminobutyric acid A receptor, alpha 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 70kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 61.6kDa according to NP_000800.2). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Cortex shows patchy staining near or at the neuronal cell membrane.. Recommended concentration, 2-4ug/ml.
Background References:
  • Ma DQ, Whitehead PL, Menold MM, Martin ER, Ashley-Koch AE, Mei H, Ritchie MD, Delong GR, Abramson RK, Wright HH, Cuccaro ML, Hussman JP, Gilbert JR, Pericak-Vance MA. Identification of significant association and gene-gene interaction of GABA receptor subunit genes in autism. Am J Hum Genet. 2005 Sep;77(3):377-88.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-EKAKRKTSKPPQE
NCBI Gene ID #:
2557
NCBI Official Name:
gamma-aminobutyric acid (GABA) A receptor, alpha 4
NCBI Official Symbol:
GABRA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000800.2
Protein GI Number:
34452723
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
.
Swissprot #:
P48169