hCAP-H Antibody

ProSci
Product Code: PSI-45-724
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-724-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
45-724 (0.01ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-724 (0.01ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
HCAP-H, NCAPH, non-SMC condensin I complex, subunit H, BRRN1, CAP-H, XCAP-H homolog, barren homolog 1, chromosome-associated protein H, condensin complex subunit 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 82.6kDa according to NP_056156.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cabello et al, Mol Biol Cell. 2001 Nov;12(11):3527-37.; PMID: 11694586). Recommended concentration: 0.01-0.03ug/ml.
Background References:
  • Torres-Rosell J, De Piccoli G, Cordon-Preciado V, Farmer S, Jarmuz A, Machin F, Pasero P, Lisby M, Haber JE, Aragon L. Anaphase onset before complete DNA replication with intact checkpoint responses. Science. 2007 Mar 9;315(5817):1411-5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TEDLSDVLVRQGD
NCBI Gene ID #:
23397
NCBI Official Name:
non-SMC condensin I complex, subunit H
NCBI Official Symbol:
NCAPH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056156.2
Protein GI Number:
25121987
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q15003