HDAC1 Antibody

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ProSci
Product Code: PSI-42-976
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-976-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-976 (0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-976 (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
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42-976 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blu
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42-976 (0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-976 (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
42-976 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blu

Documents

Further Information

Additional Names:
HDAC1, histone deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1 , reduced potassium dependency, yeast homolog-like 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Appox 55kDa band observed in Human Duodenum and Lymph Node lysates and approx 70kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1kDa according to Human NP_004955.2 and Mouse NP_032254.1). The observed 70kDa band represents the sumoylated protein according to David et al, J Biol Chem. 2002 Jun 28;277(26):23658-63; PMID: 11960997. Recommended concentration: 0.1-2ug/ml.

Immunofluorescence: Strong expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10ug/ml

Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB07186 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KPEAKGVKEEVK
NCBI Gene ID #:
3065
NCBI Official Name:
histone deacetylase 1
NCBI Official Symbol:
HDAC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Appox 55 kDa
Protein Accession #:
NP_004955.2
Protein GI Number:
13128860
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q13547