Hsd11b1 Antibody

ProSci
Product Code: PSI-42-861
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-861-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-861 (0.3ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-861 (0.3ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
Hsd11b1, hydroxysteroid (11-beta) dehydrogenase 1, 11-DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, 11-beta-hydroxysteroid dehydrogenase 1, corticosteroid 11-beta-dehydrogenase isozyme 1, short chain dehydrogenase/reductase family 26C, member 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 32kDa band observed in Mouse Lung lysates (calculated MW of 32.4kDa according to NP_032314.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-TARSEEGLQKVVSR
NCBI Gene ID #:
15483 (mouse); 25116 (rat);
NCBI Official Name:
hydroxysteroid 11-beta dehydrogenase 1
NCBI Official Symbol:
Hsd11b1
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_032314.2
Protein GI Number:
113680741
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
P50172