IREB2 Antibody

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ProSci
Product Code: PSI-42-103
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-103-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-103 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-103 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
IREB2, iron-responsive element binding protein 2, ACO3, FLJ23381, IRP2, IRP2AD, iron regulatory protein 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Liver lysates (calculated MW of 105kDa according to NP_004127.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was confirmed as a breakdown product in aged purified recombinant protein by a free sample recipient.Immunohistochemistry:In paraffin embedded Human Kidney shows preferential staining in the cytoplasm of DCT. Recommended concentration, 3-6ug/ml.
Background References:
  • Wang W, Di X, D\1Agostino RB, Torti SV, Torti FM, Excess capacity of the iron regulatory protein system. The Journal of biological chemistry 2007 Aug 282 (34): 24650-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-SIHYEGSEYKLSHGS
NCBI Gene ID #:
3658
NCBI Official Name:
iron-responsive element binding protein 2
NCBI Official Symbol:
IREB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004127.1
Protein GI Number:
41352693
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
P48200