ITGAL Antibody

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ProSci
Product Code: PSI-42-769
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-769-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-769 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-769 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-769 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-769 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
ITGAL, integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide), CD11A, LFA-1, LFA1A, LFA-1 alpha, antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide, integrin alpha L, integrin gene promoter, lymphocyte function-associated antigen 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 200kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129kDa according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-YQVRIQPSIHDHN
NCBI Gene ID #:
3683
NCBI Official Name:
integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
NCBI Official Symbol:
ITGAL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002200.2, NP_001107852.1
Protein GI Number:
167466215
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).
Swissprot #:
P20701