ITIH4 Antibody
| Code | Size | Price |
|---|
| PSI-42-059-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
ITIH4, inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein), DKFZp686G21125, H4P, IHRP, ITIHL1, PK120, inter-alpha (globulin) inhibitor H4, plasma kallikrein-sensitive glycoprotein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 150kDa band in Human Liver and Lung lysates after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 103kDa according to NP_002209.2. The 150kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 4-6ug/ml.
Background References:
- Tang Y, Kitisin K, Jogunoori W, Li C, Deng CX, Mueller SC, Ressom HW, Rashid A, He AR, Mendelson JS, Jessup JM, Shetty K, Zasloff M, Mishra B, Reddy EP, Johnson L, Mishra L. Progenitor/stem cells give rise to liver cancer due to aberrant TGF-beta and IL-6 signaling. Proc Natl Acad Sci USA. 2008 Feb 19;105(7):2445-50. Epub 2008 Feb 8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: ERLCVDPRHRQGPVN
NCBI Gene ID #:
3700
NCBI Official Name:
inter-alpha-trypsin inhibitor heavy chain family, member 4
NCBI Official Symbol:
ITIH4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002209.2
Protein GI Number:
31542984
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Innate Immunity
SPECIFICITY:
The immunizing peptide represents a region of the 35kDa chain. This chain is glycosylated giving rise to an apparent higher molecular weight.
Swissprot #:
Q14624