Klk6 Antibody

ProSci
Product Code: PSI-42-098
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-098-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-098 staining (2ug/ml) of Mouse Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

42-098 staining (2ug/ml) of Mouse Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
Klk6, kallikrein related-peptidase 6, AI849898, Bssp, Klk29, Klk7, Prss18, Prss9, neurosin, kallikrein 29, kallikrein 6, kallikrein 7, protease M, protease, serine, 18, protease, serine, 9
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 22kDa band observed in Mouse Liver lysates (calculated MW of 27.5kDa according to NP_035307.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 48kDa. Both bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Iwata A, Maruyama M, Akagi T, Hashikawa T, Kanazawa I, Tsuji S, Nukina N, Alpha-synuclein degradation by serine protease neurosin: implication for pathogenesis of synucleinopathies. Hum Mol Genet. 2003 Oct 15;12(20):2625-35. Epub 2003 Aug 19.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: KEKPGVYTDVCTHIR
NCBI Gene ID #:
19144 (mouse); 29245 (rat);
NCBI Official Name:
kallikrein related-peptidase 6
NCBI Official Symbol:
Klk6
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_035307.1
Protein GI Number:
6755180
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Neuroscience
SPECIFICITY:
.
Swissprot #:
Q91Y82