LITAF Antibody

ProSci
Product Code: PSI-46-681
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-681-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-681 (6.5ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-681 (6.5ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
LITAF, lipopolysaccharide-induced TNF factor, CMT1C, FLJ38636, MGC116698, MGC116700, MGC116701, MGC125274, MGC125275, MGC125276, PIG7, SIMPLE, TP53I7, LPS-induced TNF-alpha factor, small integral membrane protein of lysosome/late endosome, tumor protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 25kDa band in Human Lymph Node lysates and in lysates of cell lines HeLa and HepG2 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_004853.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 6.5ug/ml.
Background References:
  • Tang X, Metzger D, Leeman S, Amar S. LPS-induced TNF-alpha factor (LITAF)-deficient mice express reduced LPS-induced cytokine: Evidence for LITAF-dependent LPS signaling pathways. Proc Natl Acad Sci USA. 2006 Sep 12;103(37):13777-82. Epub 2006 Sep 5. Erratum
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-PDGKGMNPPSYYTQ
NCBI Gene ID #:
9516
NCBI Official Name:
lipopolysaccharide-induced TNF factor
NCBI Official Symbol:
LITAF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004853.2, NP_001129945.1
Protein GI Number:
65787265
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Apoptosis
SPECIFICITY:
This antibody is excpected to crossreact with both reported isoforms (NP_004853.2; NP_001129945.1).
Swissprot #:
Q99732