MAD2L1 Antibody

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ProSci
Product Code: PSI-45-853
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-853-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-853 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-853 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
MAD2L1, MAD2 mitotic arrest deficient-like 1 (yeast), MAD2, HSMAD2, mitotic arrest deficient, yeast, homolog-like 1, MAD2 (mitotic arrest deficient, yeast, homolog)-like 1, MAD2-like 1, MAD2-like protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 24kDa band observed in lysates of cell line HEK293 (calculated MW of 23.5kDa according to NP_002349.1). Recommended concentration: 2-4ug/ml. An additional band of unknown identity was also consistently observed at approx. 29kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Li Y, Benezra R. Identification of a human mitotic checkpoint gene: hsMAD2. Science. 1996 Oct 11;274(5285):246-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KVNSMVAYKIPVND
NCBI Gene ID #:
4085
NCBI Official Name:
MAD2 mitotic arrest deficient-like 1 (yeast)
NCBI Official Symbol:
MAD2L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002349.1
Protein GI Number:
4505067
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q13257