MARCH7 Antibody

ProSci
Product Code: PSI-46-686
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-686-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-686 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-686 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
MARCH7, membrane-associated ring finger (C3HC4) 7, AXO, AXOT, DKFZP586F1122, MARCH-VII, RNF177, axotrophin, membrane-associated RING-CH protein VII
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 125kDa band in Human Spleen and Tonsil lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.1kDa according to NP_073737.1. The 125kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 3.75ug/ml.
Background References:
  • Muthukumarana PA, Lyons GE, Miura Y, Thompson LH, Watson T, Green CJ, Shurey S, Hess AD, Rosengard BR, Metcalfe SM. Evidence for functional inter-relationships between FOXP3, leukaemia inhibitory factor, and axotrophin/MARCH-7 in transplantation tolerance. Int Immunopharmacol. 2006 Dec 20;6(13-14):1993-2001. Epub 2006 Oct 17.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-NFNQESEGRNT
NCBI Gene ID #:
64844
NCBI Official Name:
membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase
NCBI Official Symbol:
Mar-07
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_073737.1
Protein GI Number:
12383066
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Swissprot #:
Q9H992