MDA5 Antibody

ProSci
Product Code: PSI-45-874
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-874-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-874 (10ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

45-874 (10ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Further Information

Additional Names:
IFIH1, MDA5, MDA-5, Hlcd, MGC133047, IDDM19, melanoma differentiation associated protein-5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, interferon induced with helicase C domain 1, helicard
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:This antibody has been successfully used in Western blot on African Green Monkey: Sato S et al, Arthritis Rheum. 2009 Jul;60(7):2193-200. PMID: 19565506.Immunohistochemistry:In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration: 10ug/ml.
Background References:
  • Kang DC, Gopalkrishnan RV, Wu Q, Jankowsky E, Pyle AM, Fisher PB. mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties. Proc Natl Acad Sci USA. 2002 Jan 22;99(2):637-42.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: SNGYSTDENFRYL-C
NCBI Gene ID #:
64135
NCBI Official Name:
interferon induced with helicase C domain 1
NCBI Official Symbol:
IFIH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_071451.2
Protein GI Number:
27886568
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9BYX4