MKRN1 Antibody
| Code | Size | Price |
|---|
| PSI-42-850-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
MKRN1, makorin ring finger protein 1, FLJ21334, RNF61, E3 ubiquitin-protein ligase makorin-1, OTTHUMP00000212121, OTTHUMP00000212124, RING finger protein 61
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in fetal Mouse Brain lysates and 35kDa plus fainter 50kDa bands in adult Mouse Brain lysates (calculated MW of 52.9kDa according to NP_061280.2). The observed 35kDa band corresponds to the calculated MW of human isoform 2 (35.2kDa according to NP_001138597.1), but this size isoform has not yet been confirmed in Mouse. Recommended concentration: 1-3ug/ml. In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.3- 1ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RYEHSKPLKQEEAT
NCBI Gene ID #:
23608
NCBI Official Name:
makorin ring finger protein 1
NCBI Official Symbol:
MKRN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_038474.2, NP_001138597.1
Protein GI Number:
223468620
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1).
Swissprot #:
Q9UHC7