MOB2 Antibody

ProSci
Product Code: PSI-42-837
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-837-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 2
42-837 (0.02ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2 / 2

42-837 (0.02ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
MOB2, HCCA2, HCCA2 protein, mob2 homolog, mps one binder kinase activator-like 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 30.2kDa according to NP_001165694.1). Recommended concentration: 0.02-0.06ug/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration, 5-10ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: DERGKKVKCTAPQ
NCBI Gene ID #:
81532
NCBI Official Name:
MOB kinase activator 2
NCBI Official Symbol:
MOB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001165694.1, NP_443731.2
Protein GI Number:
288557345
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only.
Swissprot #:
Q70IA6