MSLN Antibody

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ProSci
Product Code: PSI-42-503
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-503-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-503 (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-503 (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
MSLN, mesothelin, CAK1, MPF, SMR, megakaryocyte potentiating factor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Human Placenta lysates (calculated MW of 68.1kDa according to NP_005814.2 and of 69.0kDa according to NP_037536.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CPSGKKAREIDES
NCBI Gene ID #:
10232
NCBI Official Name:
mesothelin
NCBI Official Symbol:
MSLN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005814.2, NP_037536.2
Protein GI Number:
53988378
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize isoform 1 and 2 (NP_005814.2; NP_037536.2).
Swissprot #:
Q13421