NF45 Antibody
| Code | Size | Price |
|---|
| PSI-46-677-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
ILF2, NF45, interleukin enhancer binding factor 2, 45kDa , MGC8391, PRO3063 , interleukin enhancer binding factor 2, nuclear factor of activated T-cells, 45-kDa
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 43kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1kDa according to NP_004506.2). Recommended concentration: 0.03-0.1ug/ml. This antibody has been successfully used in WB in the following papers: Ye J et al, RNA. 2017 Aug;23(8):1270-1284. PMID: 28487382. And Shamanna RA et al, Oncogene. 2013 Oct 24;32(43):5176-85 PMID: 23208500.Immunoprecipitation: This antibody was confirmed by a customer in a special IP procedure called PAR-CLIP (see for method PMID: 20644507). This data is from a previous batch, not on sale.
Background References:
- Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH. A protein interaction network for pluripotency of embryonic stem cells. Nature. 2006 Nov 16;444(7117):364-8. Epub 2006 Nov 8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-EKAYEKPPEKKEG
NCBI Gene ID #:
3608
NCBI Official Name:
interleukin enhancer binding factor 2
NCBI Official Symbol:
ILF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004506.2, NP_001254738.1
Protein GI Number:
24234747
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_004506.2; NP_001254738.1).
Swissprot #:
Q12905