KSHV ORF45 Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-228-0.1mL | 0.1mL | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 2D4A5
Regulatory Status: RUO
Target Species: Virus
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
to KSHV ORF45 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Protein ORF45
Application Note:
Western Blot:1:500 - 1,000
ELISA:Determining optimal working dilutions by titration test.
ELISA:Determining optimal working dilutions by titration test.
Background:
Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi?s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman?s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.
Background References:
- Chang Y. et al. 1994. Science. 266:1865-1869.
- Zhu FX. et al. 2002. PNAS. 99: 5573-5578.
- Zhu FX. et al. J Virol. 2003. April (77): 4221-4230.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human KSHV ORF45 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
4961474
NCBI Official Name:
virion phosphoprotein
NCBI Official Symbol:
ORF45
NCBI Organism:
Human herpesvirus 8
Protein Accession #:
F5HDE4
Protein GI Number:
550540839
Research Area:
Infectious Disease
Swissprot #:
F5HDE4
User NOte:
Optimal dilutions for each application to be determined by the researcher.