BLK Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-117-0.1mL | 0.1mL | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 9D10B7
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
BLK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
B lymphocyte kinaseMODY11
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56 kDa), belongs to the Src non-receptor tyrosine kinases family.Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking. Blk is expressed exclusively by B lymphocytes and it is thought to function in a signal transductory pathway specific to this lineage. B lymphoid expression of an active Blk mutant caused proliferation of B progenitor cells and enhanced responsiveness of these cells to interleukin 7. Thus, sustained activation of Blk induces responses normally associated with the pre-BCR.
Background References:
- Theresa Tretter, Ashley E. Ross, Dominic I. Dordai. J. Exp. Med., Dec 2003; 198: 1863.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant BLK expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
640
NCBI Official Name:
B lymphoid tyrosine kinase
NCBI Official Symbol:
BLK
NCBI Organism:
Homo sapiens
Protein Accession #:
P51451
Protein GI Number:
158936749
Research Area:
Apoptosis
Swissprot #:
P51451
User NOte:
Optimal dilutions for each application to be determined by the researcher.