BTK Monoclonal Antibody

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ProSci
Product Code: PSI-32-123
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-123-0.1mL0.1mL£560.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 7F12H4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
BTK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate.
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Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate.

Documents

Further Information

Additional Names:
AGMX1, ATK, BPK, Agammaglobulinemia tyrosine kinase, AT, XLA, IMD1, AGMX1, PSCTK1
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
Background References:
  • Yamada, N., et al. Biochem. Biophys. Res. Commun. 192: 231-240.
  • Thomas, J.D., et al. 1993. Science. 261: 355-358.
  • Tamagnone, L., et al. Oncogene 9: 3683-3688.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
695
NCBI Official Name:
Bruton agammaglobulinemia tyrosine kinase
NCBI Official Symbol:
BTK
NCBI Organism:
Homo sapiens
Protein Accession #:
Q06187
Protein GI Number:
547759
Research Area:
Signal Transduction ,Innate Immunity
Swissprot #:
Q06187
User NOte:
Optimal dilutions for each application to be determined by the researcher.