CD34 Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-132-0.1mL | 0.1mL | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: 4H5E7
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
CD34 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Hematopoietic progenitor cell antigen CD34, CD_antigen=CD34
Application Note:
Dilution
Western Blot:1:200 - 1:1,000
IF:1:25 - 1:100
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Western Blot:1:200 - 1:1,000
IF:1:25 - 1:100
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Background References:
- Sho E., et al. Arterioscler Thromb Vasc Biol. 2004: 24: 1916-21.
- Satterthwaite, A.B.,et al. Genomics.1992:12:788-794.
- Krause, D.S.,et al. 1994. Blood.84: 691-701.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant CD34 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
947
NCBI Official Name:
CD34 molecule
NCBI Official Symbol:
CD34
NCBI Organism:
Homo sapiens
Protein Accession #:
P28906
Protein GI Number:
3183511
Research Area:
Immunology ,Neuroscience
Swissprot #:
P28906
User NOte:
Optimal dilutions for each application to be determined by the researcher.