GSK3 alpha Monoclonal Antibody

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ProSci
Product Code: PSI-32-165
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-165-0.1mg0.1mg£560.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 6G12C2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
GSK3 alpha monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using GSK3a monoclonal antibody against truncated GSK3a recombinant protein and Hela cell lysate.
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Western blot analysis using GSK3a monoclonal antibody against truncated GSK3a recombinant protein and Hela cell lysate.

Documents

Further Information

Additional Names:
GSK-3 alpha,
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily?GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.
Background References:
  • Mendez P, Garcia-Segura LM. Endocrinology. 2006 Jun;147(6):3027-39. Epub 2006 Feb 23.
  • Bianchi M, De Lucchini S, et, al. Biochem J. 2005 Oct 15;391(Pt 2):359-70.
  • De Servi B, Hermani A, Medunjanin S, Mayer D. Oncogene. 2005 Jul 21;24(31):4946-55.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant GSK3 alpha expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
2931
NCBI Official Name:
glycogen synthase kinase 3 alpha
NCBI Official Symbol:
GSK3A
NCBI Organism:
Homo sapiens
Protein Accession #:
P49840
Protein GI Number:
12644292
Research Area:
Signal Transduction
Swissprot #:
P49840
User NOte:
Optimal dilutions for each application to be determined by the researcher.