ZAP70 Monoclonal Antibody

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ProSci
Product Code: PSI-32-237
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-237-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3D2A4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
ZAP70 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using Dynamin1 monoclonal antibody against truncated ZAP70 recombinant protein and Jurkat cell lysate.
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Western blot analysis using Dynamin1 monoclonal antibody against truncated ZAP70 recombinant protein and Jurkat cell lysate.

Documents

Further Information

Additional Names:
SRK, 70 kDa zeta-chain associated proteinSRK, STD, TZK, STCD, ZAP-70
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
ZAP70(zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Zap70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the zap-70 gene. ZAP-70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.
Background References:
  • Sigal Gelkop , Gerrald D. Gish, Yael Babichev J Immunol. 2005 Dec 15;175(12):8123-32.
  • Joaquim Carreras , Neus Villamor ,Lluis Colomo J Pathol. 2005 Mar;205(4):507-13.
  • Claire Hivroz Med Sci (Paris). 2005 Feb;21(2):150-5.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant ZAP70 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
7535
NCBI Official Name:
zeta-chain (TCR) associated protein kinase 70kDa
NCBI Official Symbol:
ZAP70
NCBI Organism:
Homo sapiens
Protein Accession #:
P43403
Protein GI Number:
1177044
Research Area:
Signal Transduction ,Immunology ,Innate Immunity
Swissprot #:
P43403
User NOte:
Optimal dilutions for each application to be determined by the researcher.