NLRP2 Antibody

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ProSci
Product Code: PSI-42-532
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-532-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with 42-532 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed with 42-532 (1mg/ml) in Lane B. Primary
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with 42-532 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed with 42-532 (1mg/ml) in Lane B. Primary

Documents

Further Information

Additional Names:
NLRP2, NLR family, pyrin domain containing 2, CLR19.9, FLJ20510, NALP2, NBS1, PAN1, PYPAF2, NACHT, LRR and PYD containing protein 2, NACHT, leucine rich repeat and PYD containing 2, PYRIN-Containing APAF1-like, nucleotide-binding oligomerization domain, l
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human NLRP2 (myc and DYKDDDDK tagged), a band of approx. 150kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.Purchase the +ve control! The transfected lysate used in QC is available for sale. Please email sales@everestiotech.com to request datasheet, pricing and delivery information.
Background References:
  • Ji S, Shin JE, Kim YS, Oh JE, Min BM, Choi Y, Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. Infection and immunity 2009 Mar 77 (3): 1044-52.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KNPQLIIDTEKHHP
NCBI Gene ID #:
55655
NCBI Official Name:
NLR family, pyrin domain containing 2
NCBI Official Symbol:
NLRP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060322.1
Protein GI Number:
8923473
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
Q9NX02