NLRP3 Antibody

ProSci
Product Code: PSI-42-536
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-536-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-536 (5ug/ml) staining of paraffin embedded Human peripheral blood leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

42-536 (5ug/ml) staining of paraffin embedded Human peripheral blood leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
NLRP3, NLR family, pyrin domain containing 3, AGTAVPRL, AII, AII/AVP, AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, FLJ95925, MWS, NALP3, PYPAF1, AII/AVP receptor-like, NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3, NACHT, LRR and PYD contain
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 36kDa band in Human Peripheral Blood Mononucleocytes lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the smallest calculated size of 106kDa according to NP_899632.1. The 36kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human peripheral blood leukocytes. Recommended concentration: 5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: RDLYEKAKRDEPK-C
NCBI Gene ID #:
114548
NCBI Official Name:
NLR family, pyrin domain containing 3
NCBI Official Symbol:
NLRP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004886.3, NP_899632.1, NP_001120933.1, NP_001120934.1
Protein GI Number:
34878693
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Immunology ,Apoptosis
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1). Reported variants NP_001073289.1 and NP_004886.3 represent identical protein.
Swissprot #:
Q96P20