NPR3 Antibody

ProSci
Product Code: PSI-42-909
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-909-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-909 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-909 (10ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

42-909 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-909 (10ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
NPR3, natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C), ANPRC, GUCY2B, NPRC, ANP-C, ANPR-C, NPR-C, OTTHUMP00000197048, OTTHUMP00000220980, atrial natriuretic peptide clearance receptor, atrial natriuretic peptide receptor 3, atrial natriuretic peptide receptor type C
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 52kDa band observed in Human Placenta lysates (calculated MW of 59.8kDa according to NP_000899.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of brush borders in select PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HRELREDSIRSHFS
NCBI Gene ID #:
4883
NCBI Official Name:
natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C)
NCBI Official Symbol:
NPR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 52 kDa
Protein Accession #:
NP_000899.1
Protein GI Number:
4505441
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
The immunizing peptide represents the cytoplasmic part of the protein.
Swissprot #:
P17342